Mbd3 Promotes Reprogramming of Primary Human Fibroblasts
نویسندگان
چکیده
منابع مشابه
Reprogramming fibroblasts into cardiomyocytes.
A deficiency of cardiomyocytes underlies most cases of heart failure, and scientists have long sought to repopulate the heart with new cardiomyocytes. Most attempts at remuscularization have been based on the transplantation of stem cells or their derivatives, and success has been modest to date. A recent study by Ieda and colleagues1 points to a new therapeutic strategy: reprogramming fibrobla...
متن کاملExcessive Cellular Proliferation Negatively Impacts Reprogramming Efficiency of Human Fibroblasts.
UNLABELLED The impact of somatic cell proliferation rate on induction of pluripotent stem cells remains controversial. Herein, we report that rapid proliferation of human somatic fibroblasts is detrimental to reprogramming efficiency when reprogrammed using a lentiviral vector expressing OCT4, SOX2, KLF4, and cMYC in insulin-rich defined medium. Human fibroblasts grown in this medium showed hig...
متن کاملDirect Reprogramming of Human Fibroblasts toward a Cardiomyocyte-like State
Direct reprogramming of adult somatic cells into alternative cell types has been shown for several lineages. We previously showed that GATA4, MEF2C, and TBX5 (GMT) directly reprogrammed nonmyocyte mouse heart cells into induced cardiomyocyte-like cells (iCMs) in vitro and in vivo. However, GMT alone appears insufficient in human fibroblasts, at least in vitro. Here, we show that GMT plus ESRRG ...
متن کاملReprogramming of human fibroblasts toward a cardiac fate.
Reprogramming of mouse fibroblasts toward a myocardial cell fate by forced expression of cardiac transcription factors or microRNAs has recently been demonstrated. The potential clinical applicability of these findings is based on the minimal regenerative potential of the adult human heart and the limited availability of human heart tissue. An initial but mandatory step toward clinical applicat...
متن کاملReprogramming of human fibroblasts to pluripotency with lineage specifiers.
Since the initial discovery that OCT4, SOX2, KLF4, and c-MYC overexpression sufficed for the induction of pluripotency in somatic cells, methodologies replacing the original factors have enhanced our understanding of the reprogramming process. However, unlike in mouse, OCT4 has not been replaced successfully during reprogramming of human cells. Here we report on a strategy to accomplish this re...
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ژورنال
عنوان ژورنال: International Journal of Stem Cells
سال: 2018
ISSN: 2005-5447
DOI: 10.15283/ijsc18036